Anti-Enolase antibody (Internal Region) (STJ13100520)
SPECIFICATIONS
ClonalityPolyclonal
HostGuinea Pig
ConjugationUnconjugated
ImmunogenA synthetic peptide from aa region of 260-280 of human Enolase conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
General Information
| Short Description | Guinea Pig polyclonal anti-Enolase (Internal Region) for use in WB in Human, Rat, Mouse and Marmoset samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Guinea Pig |
| Reactivity | Human/Rat/Mouse/Marmoset |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB. A dilution of 1:1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Eno1 |
| Gene ID | 13806/433182 |
| Uniprot ID | ENOA_MOUSE |
| Immunogen | A synthetic peptide from aa region of 260-280 of human Enolase conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Immunogen Region | Internal Region |
| Specificity | Specific for Enolase (alpha, beta, gamma). |
Additional Info
| Post Translational Modifications | ISGylated. Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity. |
| Function | Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. |
| Protein Name | Alpha-Enolase2-Phospho-D-Glycerate Hydro-LyaseEnolase 1Non-Neural EnolaseNne |
| Database Links | |
| Cellular Localisation | CytoplasmCell MembraneCan Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) FormEno1 Is Localized To The M-Band |
| Alternative Antibody Names | Anti-Alpha-Enolase antibodyAnti-2-Phospho-D-Glycerate Hydro-Lyase antibodyAnti-Enolase 1 antibodyAnti-Non-Neural Enolase antibodyAnti-Nne antibodyAnti-Eno1 antibodyAnti-Eno-1 antibody |
Information sourced from Uniprot.org