Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4ยฐC overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-ENO2 antibody [13E2] (STJ96968)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenSynthetic Peptide of NSE
STJ96968
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General Information

Short DescriptionMouse monoclonal anti-Gamma-enolase for use in WB, IF and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/IHC
HostMouse
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone ID13E2
IsotypeIgG1
ConjugationUnconjugated
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution RangeWB 1:2000
IHC 1:200
IF 1:200
FormulationLiquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolENO2
Gene ID2026
Uniprot IDENOG_HUMAN
ImmunogenSynthetic Peptide of NSE
SpecificityThe antibody detects endogenous NSE proteins.

Additional Info

Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Protein Name Gamma-Enolase
2-Phospho-D-Glycerate Hydro-Lyase
Enolase 2
Neural Enolase
Neuron-Specific Enolase
Nse
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Cell Membrane
Can Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) Form
Alternative Antibody Names Anti-Gamma-Enolase antibody
Anti-2-Phospho-D-Glycerate Hydro-Lyase antibody
Anti-Enolase 2 antibody
Anti-Neural Enolase antibody
Anti-Neuron-Specific Enolase antibody
Anti-Nse antibody
Anti-ENO2 antibody

Information sourced from Uniprot.org

Citations

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