• STJ70867 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70867 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70867 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Engrailed-2 antibody (C-Term) (STJ70867)

SKU:
STJ70867-100

€379.38
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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Engrailed-2 (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-1µg/ml
IF-Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EN2
Gene ID: 2020
Uniprot ID: HME2_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001418.2
Immunogen Sequence: NHSTTAKEGKSDSE
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Engrailed-2 antibody
Anti-Homeobox Protein En-2 antibody
Anti-Hu-En-2 antibody
Anti-EN2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance