| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Endo G (49-297) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ENDOG |
| Gene ID: |
2021 |
| Uniprot ID: |
NUCG_HUMAN |
| Immunogen Region: |
49-297 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human Endo G (NP_004426.2). |
| Immunogen Sequence: |
AELPPVPGGPRGPGELAKYG LPGLAQLKSRESYVLCYDPR TRGALWVVEQLRPERLRGDG DRRECDFREDDSVHAYHRAT NADYRGSGFDRGHLAAAANH RWSQKAMDDTFYLSNVAPQV PHLNQNAWNNLEKYSRSLTR SYQNVYVCTGPLFLPRTEAD GKSYVKYQVIGKNHVAVPTH FFKVLILEAAGGQIELRTYV MPNAPVDEAIPLERFLVPIE SIERASGLLFVPNILARAG |
| Post Translational Modifications | GSK3-beta-mediated dual phosphorylations at Thr-128 and Ser-288 is necessary for its interaction with YWHAG and the induction of autophagy. |
| Function | Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs. Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs. Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response. GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation. Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication. |
| Protein Name | Endonuclease G - MitochondrialEndo G |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Endonuclease G - Mitochondrial antibodyAnti-Endo G antibodyAnti-ENDOG antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
