• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ENAH Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from HepG2 cells, using ENAH Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293 cells using Mena Polyclonal Antibody diluted at 1：2000

Anti-ENAH antibody (472-521 aa) (STJ94096)

SKU:
STJ94096

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein enabled homolog (472-521 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENAH
Gene ID: 55740
Uniprot ID: ENAH_HUMAN
Immunogen Region: 472-521 aa
Specificity: Mena Polyclonal Antibody detects endogenous levels of Mena protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ENAH at the amino acid range 472-521
Post Translational Modifications NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
Function Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation.
Protein Name Protein Enabled Homolog
Database Links Reactome: R-HSA-202433
Reactome: R-HSA-376176
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Lamellipodium
Filopodium
Synapse
Cell Junction
Focal Adhesion
Targeted To The Leading Edge Of Lamellipodia And Filopodia By Mrl Family Members
Colocalizes At Filopodial Tips With A Number Of Other Proteins Including Vinculin And Zyxlin
Colocalizes With N-Wasp At The Leading Edge
Colocalizes With Gphn And Pfn At Synapses
Alternative Antibody Names Anti-Protein Enabled Homolog antibody
Anti-ENAH antibody
Anti-MENA antibody

Information sourced from Uniprot.org

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