• Western blot analysis of extracts of various cell lines, using EME1 antibody (STJ110673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using EME1 antibody (STJ110673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EME1 antibody (1-320) (STJ110673)

SKU:
STJ110673

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EME1 (1-320) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EME1
Gene ID: 146956
Uniprot ID: EME1_HUMAN
Immunogen Region: 1-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EME1 (NP_689676.2).
Immunogen Sequence: MALKKSSPSLDSGDSDSEEL PTFAFLKKEPSSTKRRQPER EEKIVVVDISDCEASCPPAP ELFSPPVPEIAETVTQTQPV RLLSSESEDEEEFIPLAQRL TCKFLTHKQLSPEDSSSPVK SVLDHQNNEGASCDWKKPFP KIPEVPLHDTPERSAADNKD LILDPCCQLPAYLSTCPGQS SSLAVTKTNSDILPPQKKTK PSQKVQGRGSHGCRQQRQAR QKESTLRRQERKNAALVTR
Function Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
Protein Name Crossover Junction Endonuclease Eme1
Mms4 Homolog
Hmms4
Database Links Reactome: R-HSA-5693568
Reactome: R-HSA-6783310
Cellular Localisation Nucleus
Nucleolus
Recruited To Regions Of Dna Damage In S-Phase Cells
Alternative Antibody Names Anti-Crossover Junction Endonuclease Eme1 antibody
Anti-Mms4 Homolog antibody
Anti-Hmms4 antibody
Anti-EME1 antibody
Anti-MMS4 antibody

Information sourced from Uniprot.org

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