Anti-ELP3 antibody (STJ190801)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-Elongator complex protein 3 acetyltransferase for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ELP3 |
| Gene ID | 55140 |
| Uniprot ID | ELP3_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | ELP3 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Tyrosine-phosphorylated.phosphorylation on Tyr-202 does not affect elongator complex integrity or ELP3 protein stability. Also serine/threonine-phosphorylated. |
| Function | Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins.such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex. |
| Protein Name | Elongator Complex Protein 3Help3Trna Uridine(34 Acetyltransferase |
| Database Links | Reactome: R-HSA-3214847 |
| Cellular Localisation | CytoplasmNucleusIsoform 1: NucleusIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Elongator Complex Protein 3 antibodyAnti-Help3 antibodyAnti-Trna Uridine(34 Acetyltransferase antibodyAnti-ELP3 antibody |
Information sourced from Uniprot.org