Anti-ELK1 antibody (408-428 aa) [R06-5J2] (STJA0011273)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human ELK1
General Information
| Short Description | Rabbit monoclonal anti-ELK1 (408-428 aa) for use in WB, IHC-P, IP and ChIP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ChIP |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R06-5J2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IP 1:20ChIP 1:20 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ELK1 |
| Gene ID | 2002 |
| Uniprot ID | ELK1_HUMAN |
| Immunogen | A synthetic peptide of human ELK1 |
| Immunogen Region | 408-428 aa |
Additional Info
| Tissue Specificity | Lung and testis. |
| Post Translational Modifications | Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention. On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1. Phosphorylated by PRP4K at Thr-417.phosphorylation activation ELK1 transcriptional activity. |
| Function | Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation. |
| Protein Name | Ets Domain-Containing Protein Elk-1 |
| Database Links | Reactome: R-HSA-198753Reactome: R-HSA-9031628Reactome: R-HSA-9609690Reactome: R-HSA-9634638 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets Domain-Containing Protein Elk-1 antibodyAnti-ELK1 antibody |
Information sourced from Uniprot.org