Anti-EIF6 antibody (180-260 aa) (STJ190159)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 180-260
General Information
| Short Description | Rabbit polyclonal anti-Eukaryotic translation initiation factor 6GCN homolog (180-260 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF6 |
| Gene ID | 3692 |
| Uniprot ID | IF6_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 180-260 |
| Immunogen Region | 180-260 aa |
| Specificity | IF6 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export. Ufmylated by UFL1. |
| Function | Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth. |
| Protein Name | Eukaryotic Translation Initiation Factor 6Eif-6B(2gcn HomologB4 Integrin InteractorCabP27(Bbp |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusNucleolusShuttles Between Cytoplasm And Nucleus/Nucleolus |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 6 antibodyAnti-Eif-6 antibodyAnti-B(2gcn Homolog antibodyAnti-B4 Integrin Interactor antibodyAnti-Cab antibodyAnti-P27(Bbp antibodyAnti-EIF6 antibodyAnti-EIF3A antibodyAnti-ITGB4BP antibodyAnti-OK antibodyAnti-SW-cl.27 antibody |
Information sourced from Uniprot.org