• Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-eIF5A/eIF5A2 antibody (STJ194756)

SKU:
STJ194756

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Eukaryotic translation initiation factor 5A-1 and Eukaryotic translation initiation factor 5A-2 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA-Pep 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF5A2
EIF5A
Gene ID: 56648
1984
Uniprot ID: IF5A2_HUMAN
IF5A1_HUMAN
Specificity: This antibody detects endogenous levels of human eIF5A/eIF5A2
Immunogen: Synthesized peptide derived from the human eIF5A/eIF5A2
Tissue Specificity Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
Post Translational Modifications Acetylated. Deacetylated by SIRT2. eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Database Links Reactome: R-HSA-204626
Cellular Localisation Cytoplasm
Nucleus
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Nuclear Pore Complex
Hypusine Modification Promotes The Nuclear Export And Cytoplasmic Localization And There Was A Dynamic Shift In The Localization From Predominantly Cytoplasmic To Primarily Nuclear Under Apoptotic Inducing Conditions
Protein Name Eukaryotic Translation Initiation Factor 5a-1
Eif-5a-1
Eif-5a1
Eukaryotic Initiation Factor 5a Isoform 1
Eif-5a
Rev-Binding Factor
Eif-4d
Alternative Names Anti-Eukaryotic Translation Initiation Factor 5a-1 antibody
Anti-Eif-5a-1 antibody
Anti-Eif-5a1 antibody
Anti-Eukaryotic Initiation Factor 5a Isoform 1 antibody
Anti-Eif-5a antibody
Anti-Rev-Binding Factor antibody
Anti-Eif-4d antibody
Anti-EIF5A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance