Anti-EIF4G1 antibody (551-650 aa) [SRM5271] (STJ11107173)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal EIF4G1 (551-650 aa) antibody for use in WB, IHC-P and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5271 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF4G1 |
| Gene ID | 1981 |
| Uniprot ID | IF4G1_HUMAN |
| Immunogen Region | 551-650 aa |
| Immunogen Sequence | GSNPGPESEGSGVPPRPEEA DETWDSKEDKIHNAENIQPG EQKYEYKSDQWKPLNLEEKK RYDREFLLGFQFIFASMQKP EGLPHISDVVLDKANKTPLR |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 551-650 of human EIF4G1 (NP_937884.2). |
Additional Info
| Post Translational Modifications | Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1. Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription. |
| Function | Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation. |
| Protein Name | Eukaryotic Translation Initiation Factor 4 Gamma 1Eif-4-Gamma 1Eif-4g 1Eif-4g1P220 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-156827Reactome: R-HSA-166208Reactome: R-HSA-429947Reactome: R-HSA-450408Reactome: R-HSA-72649Reactome: R-HSA-72662Reactome: R-HSA-72702Reactome: R-HSA-72706Reactome: R-HSA-9010553Reactome: R-HSA-975956Reactome: R-HSA-975957Reactome: R-HSA-9820841Reactome: R-HSA-9820865 |
| Cellular Localisation | CytoplasmNucleusStress Granule |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4 Gamma 1 antibodyAnti-Eif-4-Gamma 1 antibodyAnti-Eif-4g 1 antibodyAnti-Eif-4g1 antibodyAnti-P220 antibodyAnti-EIF4G1 antibodyAnti-EIF4F antibodyAnti-EIF4G antibodyAnti-EIF4GI antibody |
Information sourced from Uniprot.org