| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/IP/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Gene Symbol: |
EIF4B |
| Gene ID: |
1975 |
| Uniprot ID: |
IF4B_HUMAN |
| Immunogen Region: |
352-611 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2). |
| Immunogen Sequence: |
SQSTRAASIFGGAKPVDTAA REREVEERLQKEQEKLQRQL DEPKLERRPRERHPSWRSEE TQERERSRTGSESSQTGTST TSSRNARRRESEKSLENETL NKEEDCHSPTSKPPKPDQPL KVMPAPPPKENAWVKRSSNP PARSQSSDTEQQSPTSGGGK VAPAQPSEEGPGRKDENKVD GMNAPKGQTGNSSRGPGDGG NRDHWKESDRKDGKKDQDSR SAPEPKKPEENPASKFSSA |
| Post Translational Modifications | Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex. In response to mTORC1 activation, RPS6KA1-mediated phosphorylation at 'Ser-406' and 'Ser-422' stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function. |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Protein Name | Eukaryotic Translation Initiation Factor 4bEif-4b |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-166208Reactome: R-HSA-429947Reactome: R-HSA-72649Reactome: R-HSA-72662Reactome: R-HSA-72702Reactome: R-HSA-72706Reactome: R-HSA-9820841Reactome: R-HSA-9820865 |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4b antibodyAnti-Eif-4b antibodyAnti-EIF4B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
