| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EIF3K (1-218) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EIF3K |
| Gene ID: |
27335 |
| Uniprot ID: |
EIF3K_HUMAN |
| Immunogen Region: |
1-218 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human EIF3K (NP_037366.1). |
| Immunogen Sequence: |
MAMFEQMRANVGKLLKGIDR YNPENLATLERYVETQAKEN AYDLEANLAVLKLYQFNPAF FQTTVTAQILLKALTNLPHT DFTLCKCMIDQAHQEERPIR QILYLGDLLETCHFQAFWQA LDENMDLLEGITGFEDSVRK FICHVVGITYQHIDRWLLAE MLGDLSDSQLKVWMSKYGWS ADESGQIFICSQEESIKPKN IVEKIDFDSVSSIMASSQ |
| Tissue Specificity | Ubiquitous, with the highest levels of expression in brain, testis and kidney. |
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit KEif3kEukaryotic Translation Initiation Factor 3 Subunit 12Muscle-Specific Gene M9 ProteinPlac-24Eif-3 P25Eif-3 P28 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 3 Subunit K antibodyAnti-Eif3k antibodyAnti-Eukaryotic Translation Initiation Factor 3 Subunit 12 antibodyAnti-Muscle-Specific Gene M9 Protein antibodyAnti-Plac-24 antibodyAnti-Eif-3 P25 antibodyAnti-Eif-3 P28 antibodyAnti-EIF3K antibodyAnti-EIF3S12 antibodyAnti-ARG134 antibodyAnti-HSPC029 antibodyAnti-MSTP001 antibodyAnti-PTD001 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit polyclonal antibody (STJ27659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/101287/388854/STJ27659_1__09600.1713158416.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit polyclonal antibody (STJ27659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
