Anti-EIF3E antibody (C-Term) (STJ72427)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-EIF3E (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Dog/Cow/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 50kDa band observed in lysates of cell line Daudi (calculated MW of 52.2kDa according to NP_001559.1). Recommended concentration: 0.03-0.1µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF3E |
| Gene ID | 3646 |
| Uniprot ID | EIF3E_HUMAN |
| Accession Number | NP_001559.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | KLNQNSRSEAPN |
Additional Info
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Required for nonsense-mediated mRNA decay (NMD).may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit EEif3eEukaryotic Translation Initiation Factor 3 Subunit 6Viral Integration Site Protein Int-6 HomologEif-3 P48 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | CytoplasmNucleusPml Body |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 3 Subunit E antibodyAnti-Eif3e antibodyAnti-Eukaryotic Translation Initiation Factor 3 Subunit 6 antibodyAnti-Viral Integration Site Protein Int-6 Homolog antibodyAnti-Eif-3 P48 antibodyAnti-EIF3E antibodyAnti-EIF3S6 antibodyAnti-INT6 antibody |
Information sourced from Uniprot.org