Anti-EIF3E antibody (1-445) (STJ27400)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EIF3E (1-445) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF3E |
| Gene ID | 3646 |
| Uniprot ID | EIF3E_HUMAN |
| Immunogen Region | 1-445 |
| Immunogen Sequence | MAEYDLTTRIAHFLDRHLVF PLLEFLSVKEIYNEKELLQG KLDLLSDTNMVDFAMDVYKN LYSDDIPHALREKRTTVVAQ LKQLQAETEPIVKMFEDPET TRQMQSTRDGRMLFDYLADK HGFRQEYLDTLYRYAKFQYE CGNYSGAAEYLYFFRVLVPA TDRNALSSLWGKLASEILMQ NWDAAMEDLTRLKETIDNNS VSSPLQSLQQRTWLIHWSLF VFFNHPKGRDNIIDLFLYQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-445 of human eIF3e (NP_001559.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. |
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Required for nonsense-mediated mRNA decay (NMD).may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit EEif3eEukaryotic Translation Initiation Factor 3 Subunit 6Viral Integration Site Protein Int-6 HomologEif-3 P48 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | CytoplasmNucleusPml Body |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 3 Subunit E antibodyAnti-Eif3e antibodyAnti-Eukaryotic Translation Initiation Factor 3 Subunit 6 antibodyAnti-Viral Integration Site Protein Int-6 Homolog antibodyAnti-Eif-3 P48 antibodyAnti-EIF3E antibodyAnti-EIF3S6 antibodyAnti-INT6 antibody |
Information sourced from Uniprot.org