• Immunofluorescence analysis of NIH/3T3 cells using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of EIF2B2 in paraffin-embedded mouse brain using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EIF2B2 in paraffin-embedded human tonsil using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EIF2B2 in paraffin-embedded rat lung using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using EIF2B2 Rabbit polyclonal antibody (STJ29107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2B2 antibody (1-120) (STJ29107)

SKU:
STJ29107

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF2B2 (1-120) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B2
Gene ID: 8892
Uniprot ID: EI2BB_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human EIF2B2 (NP_055054.1).
Immunogen Sequence: MPGSAAKGSELSERIESFVE TLKRGGGPRSSEEMARETLG LLRQIITDHRWSNAGELMEL IRREGRRMTAAQPSETTVGN MVRRVLKIIREEYGRLHGRS DESDQQESLHKLLTSGGLNE
Function Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Protein Name Translation Initiation Factor Eif-2b Subunit Beta
S20i15
S20iii15
Eif-2b Gdp-Gtp Exchange Factor Subunit Beta
Database Links Reactome: R-HSA-72731
Alternative Antibody Names Anti-Translation Initiation Factor Eif-2b Subunit Beta antibody
Anti-S20i15 antibody
Anti-S20iii15 antibody
Anti-Eif-2b Gdp-Gtp Exchange Factor Subunit Beta antibody
Anti-EIF2B2 antibody
Anti-EIF2BB antibody

Information sourced from Uniprot.org

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