| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EIF2AK4 (1-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EIF2AK4 |
| Gene ID: |
440275 |
| Uniprot ID: |
E2AK4_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCN2 (NP_001013725.2). |
| Immunogen Sequence: |
MAGGRGAPGRGRDEPPESYP QRQDHELQALEAIYGADFQD LRPDACGPVKEPPEINLVLY PQGLTGEEVYVKVDLRVKCP PTYPDVVPEIELKNAKGLSN ESVNLLKSRLEELAKKHCGE VMIFELAYHVQSFLSEHNKP PPKSFHEEMLERRAQEEQQR LLEAKRKEEQEQREILHEIQ RRKEEIKEEKKRKEMAKQER LEIASLSNQDHTSKKDPGGH RTAAILHGGSPDFVGNGKH |
| Tissue Specificity | Widely expressed. Expressed in lung, smooth muscle cells and macrophages. |
| Post Translational Modifications | Autophosphorylated.autophosphorylation on Thr-899 is increased upon amino acid starvation and in UV irradiation cells and inhibited in presence of IMPACT. |
| Function | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability. Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs. Required for the translational induction of protein kinase PRKCH following amino acid starvation. Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation. Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory. Plays a role in neurite outgrowth inhibition. Plays a proapoptotic role in response to glucose deprivation. Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways. Plays a role in the antiviral response against alphavirus infection.impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication. (Microbial infection) Plays a role in modulating the adaptive immune response to yellow fever virus infection.promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation. |
| Protein Name | Eif-2-Alpha Kinase Gcn2Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4Gcn2-Like Protein |
| Database Links | Reactome: R-HSA-9633012 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eif-2-Alpha Kinase Gcn2 antibodyAnti-Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4 antibodyAnti-Gcn2-Like Protein antibodyAnti-EIF2AK4 antibodyAnti-GCN2 antibodyAnti-KIAA1338 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
