• Western blot analysis of the lysates from HUVECcells using EID1 antibody.
  • Western blot analysis of lysates from HeLa cells, using EID1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of A549 cells, using EID1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using EID-1 Polyclonal Antibody

Anti-EID1 antibody (71-120 aa) (STJ92867)

SKU:
STJ92867

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EP300-interacting inhibitor of differentiation 1 (71-120 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EID1
Gene ID: 23741
Uniprot ID: EID1_HUMAN
Immunogen Region: 71-120 aa
Specificity: EID-1 Polyclonal Antibody detects endogenous levels of EID-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human EID1 at the amino acid range 71-120
Post Translational Modifications Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.
Function Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
Protein Name Ep300-Interacting Inhibitor Of Differentiation 1
21 Kda Prb-Associated Protein
Crebbp/Ep300 Inhibitory Protein 1
E1a-Like Inhibitor Of Differentiation 1
Eid-1
Cellular Localisation Nucleus
Cytoplasm
May Shuttle Between Nucleus And Cytoplasm
Alternative Antibody Names Anti-Ep300-Interacting Inhibitor Of Differentiation 1 antibody
Anti-21 Kda Prb-Associated Protein antibody
Anti-Crebbp/Ep300 Inhibitory Protein 1 antibody
Anti-E1a-Like Inhibitor Of Differentiation 1 antibody
Anti-Eid-1 antibody
Anti-EID1 antibody
Anti-C15orf3 antibody
Anti-CRI1 antibody
Anti-RBP21 antibody
Anti-PNAS-22 antibody
Anti-PTD014 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance