Anti-EHMT2 antibody (STJA0037495)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein
General Information
| Short Description | Rabbit monoclonal anti-EHMT2 for use in WB, IHC-F, IHC-P, ICC, IF, FC and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/FC/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB-1:500-1:1000IHC-P-1:200-1:500ICC/IF-1:500-1:1000FC-1:50-1:100IP-1:20-1:50 |
| Formulation | Liquid in 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | EHMT2 |
| Gene ID | 10919 |
| Uniprot ID | EHMT2_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. |
| Post Translational Modifications | Methylated at Lys-185.automethylated. |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. |
| Protein Name | Histone-Lysine N-Methyltransferase Ehmt2Euchromatic Histone-Lysine N-Methyltransferase 2Hla-B-Associated Transcript 8Histone H3-K9 Methyltransferase 3H3-K9-Hmtase 3Lysine N-Methyltransferase 1cProtein G9a |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-3214841Reactome: R-HSA-427389Reactome: R-HSA-6804760Reactome: R-HSA-73762Reactome: R-HSA-8853884Reactome: R-HSA-8953750Reactome: R-HSA-9843970 |
| Cellular Localisation | NucleusChromosomeAssociates With Euchromatic RegionsDoes Not Associate With Heterochromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Ehmt2 antibodyAnti-Euchromatic Histone-Lysine N-Methyltransferase 2 antibodyAnti-Hla-B-Associated Transcript 8 antibodyAnti-Histone H3-K9 Methyltransferase 3 antibodyAnti-H3-K9-Hmtase 3 antibodyAnti-Lysine N-Methyltransferase 1c antibodyAnti-Protein G9a antibodyAnti-EHMT2 antibodyAnti-BAT8 antibodyAnti-C6orf30 antibodyAnti-G9A antibodyAnti-KMT1C antibodyAnti-NG36 antibody |
Information sourced from Uniprot.org