Anti-EGLN3 antibody (STJA0007108)

SKU:
STJA0007108

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Prolyl hydroxylase EGLN3 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EGLN3
Gene ID: 112399
Uniprot ID: EGLN3_HUMAN
Specificity: This antibody detects endogenous levels of PHD3 at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from human PHD3
Post Translational Modifications Ubiquitinated by SIAH1 and/or SIAH2 in response to the unfolded protein response (UPR), leading to its degradation.
Function Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as PKM, TELO2, ATF4 and HIF1A. Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4 (Probable).
Protein Name Prolyl Hydroxylase Egln3
Egl Nine Homolog 3
Hph-1
Hypoxia-Inducible Factor Prolyl Hydroxylase 3
Hif-Ph3
Hif-Prolyl Hydroxylase 3
Hph-3
Prolyl Hydroxylase Domain-Containing Protein 3
Phd3
Database Links Reactome: R-HSA-1234176
Cellular Localisation Nucleus
Cytoplasm
Colocalizes With Wdr83 In The Cytoplasm
Alternative Antibody Names Anti-Prolyl Hydroxylase Egln3 antibody
Anti-Egl Nine Homolog 3 antibody
Anti-Hph-1 antibody
Anti-Hypoxia-Inducible Factor Prolyl Hydroxylase 3 antibody
Anti-Hif-Ph3 antibody
Anti-Hif-Prolyl Hydroxylase 3 antibody
Anti-Hph-3 antibody
Anti-Prolyl Hydroxylase Domain-Containing Protein 3 antibody
Anti-Phd3 antibody
Anti-EGLN3 antibody

Information sourced from Uniprot.org

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