• Immunoprecipitation analysis of 300 Mu g extracts of NTH-3T3 cells using 3 Mu g EFTUD2 Rabbit polyclonal antibody (STJ29120). Western blot was performed from the immunoprecipitate using EFTUD2 Rabbit polyclonal antibody (STJ29120) at a dilition of 1:2000.
  • Immunofluorescence analysis of L929 cells using EFTUD2 Rabbit polyclonal antibody (STJ29120) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using EFTUD2 Rabbit polyclonal antibody (STJ29120) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using EFTUD2 Rabbit polyclonal antibody (STJ29120) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of EFTUD2 in paraffin-embedded mouse brain tissue using EFTUD2 Rabbit polyclonal antibody (STJ29120) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EFTUD2 in paraffin-embedded rat brain tissue using EFTUD2 Rabbit polyclonal antibody (STJ29120) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using EFTUD2 Rabbit polyclonal antibody (STJ29120) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EFTUD2 antibody (1-205) (STJ29120)

SKU:
STJ29120

€140.63 - €655.63
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:100
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EFTUD2
Gene ID: 9343
Uniprot ID: U5S1_HUMAN
Immunogen Region: 1-205
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).
Immunogen Sequence: MDTDLYDEFGNYIGPELDSD EDDDELGRETKDLDEMDDDD DDDDVGDHDDDHPGMEVVLH EDKKYYPTAEEVYGPEVETI VQEEDTQPLTEPIIKPVKTK KFTLMEQTLPVTVYEMDFLA DLMDNSELIRNVTLCGHLHH GKTCFVDCLIEQTHPEIRKR YDQDLCYTDILFTEQERGVG IKSTPVTVVLPDTKGKSYLF NIMDT
Function Required for pre-mRNA splicing as component of the spliceosome, including pre-catalytic, catalytic and post-catalytic spliceosomal complexes. Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Protein Name 116 Kda U5 Small Nuclear Ribonucleoprotein Component
Elongation Factor Tu Gtp-Binding Domain-Containing Protein 2
Snu114 Homolog
Hsnu114
U5 Snrnp-Specific Protein - 116 Kda
U5-116 Kda
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Alternative Antibody Names Anti-116 Kda U5 Small Nuclear Ribonucleoprotein Component antibody
Anti-Elongation Factor Tu Gtp-Binding Domain-Containing Protein 2 antibody
Anti-Snu114 Homolog antibody
Anti-Hsnu114 antibody
Anti-U5 Snrnp-Specific Protein - 116 Kda antibody
Anti-U5-116 Kda antibody
Anti-EFTUD2 antibody
Anti-KIAA0031 antibody
Anti-SNRP116 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance