Anti-EF-1 Alpha 1/2 antibody (N-Term) (STJ96859)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the N-terminal region of human EF-1 Alpha 1/2.
General Information
| Short Description | Rabbit polyclonal anti-Elongation factor 1-alpha 1 and Elongation factor 1-alpha 2 and Putative elongation factor 1-alpha-like 3 (N-Term) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and r |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EEF1A2EEF1A1P51917NA1915EF1A2_HUMANEF1A3_HUMANEF1A1_HUMAN |
| Immunogen | Synthesized peptide derived from the N-terminal region of human EF-1 Alpha 1/2. |
| Immunogen Region | N-Term |
| Specificity | EF-1 Alpha 1/2 Polyclonal Antibody detects endogenous levels of EF-1 Alpha 1/2 protein. |
Additional Info
| Post Translational Modifications | ISGylated. Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2. Trimethylated at Lys-79 by EEF1AKMT1. Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation. Trimethylated at Lys-318 by EEF1AKMT2. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4.trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at Gly-2 by EEF1AKNMT. Mono- and dimethylated at Lys-55 by EEF1AKNMT.dimethylated form is predominant. |
| Function | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. |
| Protein Name | Elongation Factor 1-Alpha 1Ef-1-Alpha-1Elongation Factor TuEf-TuEukaryotic Elongation Factor 1 A-1Eef1a-1Leukocyte Receptor Cluster Member 7 |
| Database Links | Reactome: R-HSA-156842Reactome: R-HSA-156902Reactome: R-HSA-3371511Reactome: R-HSA-6798695Reactome: R-HSA-8876725Reactome: R-HSA-9613829 |
| Cellular Localisation | CytoplasmNucleusNucleolusCell MembraneColocalizes With Dlc1 At Actin-Rich Regions In The Cell PeripheryTranslocates Together With Zpr1 From The Cytoplasm To The Nucleus And Nucleolus After Treatment With MitogensLocalization At The Cell Membrane Depends On Eef1a1 Phosphorylation Status And The Presence Of Ppp1r16b |
| Alternative Antibody Names | Anti-Elongation Factor 1-Alpha 1 antibodyAnti-Ef-1-Alpha-1 antibodyAnti-Elongation Factor Tu antibodyAnti-Ef-Tu antibodyAnti-Eukaryotic Elongation Factor 1 A-1 antibodyAnti-Eef1a-1 antibodyAnti-Leukocyte Receptor Cluster Member 7 antibodyAnti-EEF1A1 antibodyAnti-EEF1A antibodyAnti-EF1A antibodyAnti-LENG7 antibody |
Information sourced from Uniprot.org