Anti-EEF2 antibody (759-858) [S2MR] (STJ11102532)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal EEF2 (759-858) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:100-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EEF2 |
| Gene ID | 1938 |
| Uniprot ID | EF2_HUMAN |
| Immunogen Region | 759-858 |
| Immunogen Sequence | IYGVLNRKRGHVFEESQVAG TPMFVVKAYLPVNESFGFTA DLRSNTGGQAFPQCVFDHWQ ILPGDPFDNSSRPSQVVAET RKRKGLKEGIPALDNFLDKL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 759-858 of human eEF2 (P13639). |
Additional Info
| Post Translational Modifications | Phosphorylation by EF-2 kinase completely inactivates EF-2.it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. Diphthamide is 2-3-carboxyamido-3-(trimethyl-ammonio)propylhistidine. (Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated. Proteolytically processed at two sites following phosphorylation by CSK. SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage. |
| Function | Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. |
| Protein Name | Elongation Factor 2Ef-2 |
| Database Links | Reactome: R-HSA-156902Reactome: R-HSA-5336415Reactome: R-HSA-5358493Reactome: R-HSA-6798695Reactome: R-HSA-8876725 |
| Cellular Localisation | CytoplasmNucleusPhosphorylation By Csk Promotes Cleavage And Sumoylation-Dependent Nuclear Translocation Of The C-Terminal Cleavage Product |
| Alternative Antibody Names | Anti-Elongation Factor 2 antibodyAnti-Ef-2 antibodyAnti-EEF2 antibodyAnti-EF2 antibody |
Information sourced from Uniprot.org