• Immunofluorescence analysis of NIH/3T3 cells using EEF1B2 antibody (STJ28663) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using EEF1B2 Antibody (STJ28663) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using EEF1B2 Antibody (STJ28663) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spinal cord using EEF1B2 Antibody (STJ28663) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (STJ28663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (STJ28663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EEF1B2 antibody (1-225) (STJ28663)

SKU:
STJ28663

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EEF1B2 (1-225) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF1B2
Gene ID: 1933
Uniprot ID: EF1B_HUMAN
Immunogen Region: 1-225
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1).
Immunogen Sequence: MGFGDLKSPAGLQVLNDYLA DKSYIEGYVPSQADVAVFEA VSSPPPADLCHALRWYNHIK SYEKEKASLPGVKKALGKYG PADVEDTTGSGATDSKDDDD IDLFGSDDEEESEEAKRLRE ERLAQYESKKAKKPALVAKS SILLDVKPWDDETDMAKLEE CVRSIQADGLVWGSSKLVPV GYGIKKLQIQCVVEDDKVGT DMLEEQITAFEDYVQSMDVA AFNKI
Post Translational Modifications Phosphorylation affects the GDP/GTP exchange rate.
Function EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
Protein Name Elongation Factor 1-Beta
Ef-1-Beta
Database Links Reactome: R-HSA-156842
Alternative Antibody Names Anti-Elongation Factor 1-Beta antibody
Anti-Ef-1-Beta antibody
Anti-EEF1B2 antibody
Anti-EEF1B antibody
Anti-EF1B antibody

Information sourced from Uniprot.org

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