• STJ72917 (0. 5µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ72917 (0. 5µg/ml) staining of Mouse (A+C) and Rat (B+D) Skeletal Muscle (A+B) and Heart (C+D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EEF1A2 antibody (Internal) (STJ72917)

SKU:
STJ72917-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EEF1A2 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.5-2µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EEF1A2
Gene ID: 1917
Uniprot ID: EF1A2_HUMAN
Immunogen Region: Internal
Accession Number: NP_001949.1
Immunogen Sequence: NPATVPFVPIS
Post Translational Modifications Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4.trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at the N-terminus by METTL13. Mono- and dimethylated at Lys-55 by METTL13.dimethylated form is predominant.
Function This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Protein Name Elongation Factor 1-Alpha 2
Ef-1-Alpha-2
Eukaryotic Elongation Factor 1 A-2
Eef1a-2
Statin-S1
Database Links Reactome: R-HSA-156842
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Elongation Factor 1-Alpha 2 antibody
Anti-Ef-1-Alpha-2 antibody
Anti-Eukaryotic Elongation Factor 1 A-2 antibody
Anti-Eef1a-2 antibody
Anti-Statin-S1 antibody
Anti-EEF1A2 antibody
Anti-EEF1AL antibody
Anti-STN antibody

Information sourced from Uniprot.org

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