Anti-EEF1A2 antibody (184-463) (STJ111156)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EEF1A2 (184-463) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EEF1A2 |
| Gene ID | 1917 |
| Uniprot ID | EF1A2_HUMAN |
| Immunogen Region | 184-463 |
| Immunogen Sequence | NPATVPFVPISGWHGDNMLE PSPNMPWFKGWKVERKEGNA SGVSLLEALDTILPPTRPTD KPLRLPLQDVYKIGGIGTVP VGRVETGILRPGMVVTFAPV NITTEVKSVEMHHEALSEAL PGDNVGFNVKNVSVKDIRRG NVCGDSKSDPPQEAAQFTSQ VIILNHPGQISAGYSPVIDC HTAHIACKFAELKEKIDRRS GKKLEDNPKSLKSGDAAIVE MVPGKPMCVESFSQYPPLG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 184-463 of human EEF1A2 (NP_001949.1). |
Additional Info
| Tissue Specificity | Brain, heart, and skeletal muscle. |
| Post Translational Modifications | Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4.trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at the N-terminus by METTL13. Mono- and dimethylated at Lys-55 by METTL13.dimethylated form is predominant. |
| Function | Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. |
| Protein Name | Elongation Factor 1-Alpha 2Ef-1-Alpha-2Eukaryotic Elongation Factor 1 A-2Eef1a-2Statin-S1 |
| Database Links | Reactome: R-HSA-156842 |
| Cellular Localisation | Endoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-Elongation Factor 1-Alpha 2 antibodyAnti-Ef-1-Alpha-2 antibodyAnti-Eukaryotic Elongation Factor 1 A-2 antibodyAnti-Eef1a-2 antibodyAnti-Statin-S1 antibodyAnti-EEF1A2 antibodyAnti-EEF1AL antibodyAnti-STN antibody |
Information sourced from Uniprot.org