• STJ73639 (0. 5µg/ml) staining of HEK293 (A) , HeLa (B) and (1ug/ml) NIH3T3 (C) Nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73639 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73639 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73639 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-EDF1/MBF1 antibody (N-Term) (STJ73639)

SKU:
STJ73639-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EDF1/MBF1 (N-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
IF-Strong expression of the protein seen in the nuclei of A431 and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EDF1
Gene ID: 8721
Uniprot ID: EDF1_HUMAN
Immunogen Region: N-Term
Accession Number: NP_003783.1; NP_694880.1; NP_001268226.1; NP_001268227.1; NP_001268228.1
Immunogen Sequence: AESDWDTVTVLRK
Post Translational Modifications Phosphorylated (by PKA and PKC).
Function Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
Protein Name Endothelial Differentiation-Related Factor 1
Edf-1
Multiprotein-Bridging Factor 1
Mbf1
Cellular Localisation Cytoplasm
Nucleus
Also Nuclear Upon Binding To Nr5a1 And Treatment Of Cells With Tpa Or Forskolin
Alternative Antibody Names Anti-Endothelial Differentiation-Related Factor 1 antibody
Anti-Edf-1 antibody
Anti-Multiprotein-Bridging Factor 1 antibody
Anti-Mbf1 antibody
Anti-EDF1 antibody

Information sourced from Uniprot.org

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