Anti-EDF1 antibody (Internal) (STJ73046)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-EDF1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration: 0.3-1µg/ml.IHC: In paraffin embedded Human |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | EDF1 |
| Gene ID | 8721 |
| Uniprot ID | EDF1_HUMAN |
| Accession Number | NP_003783.1; NP_694880.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | NKQHSITKNTAKLDR |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_003783.1; NP_694880.1). |
Additional Info
| Post Translational Modifications | Phosphorylated (by PKA and PKC). |
| Function | Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. |
| Protein Name | Endothelial Differentiation-Related Factor 1Edf-1Multiprotein-Bridging Factor 1Mbf1 |
| Cellular Localisation | CytoplasmNucleusAlso Nuclear Upon Binding To Nr5a1 And Treatment Of Cells With Tpa Or Forskolin |
| Alternative Antibody Names | Anti-Endothelial Differentiation-Related Factor 1 antibodyAnti-Edf-1 antibodyAnti-Multiprotein-Bridging Factor 1 antibodyAnti-Mbf1 antibodyAnti-EDF1 antibody |
Information sourced from Uniprot.org