• Western blot analysis of various lysates using ECI2 Rabbit polyclonal antibody (STJ115039) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ECI2 antibody (1-364) (STJ115039)

SKU:
STJ115039

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECI2 (1-364) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECI2
Gene ID: 10455
Uniprot ID: ECI2_HUMAN
Immunogen Region: 1-364
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human ECI2 (NP_006108.2).
Immunogen Sequence: MNRTAMRASQKDFENSMNQV KLLKKDPGNEVKLKLYALYK QATEGPCNMPKPGVFDLINK AKWDAWNALGSLPKEAARQN YVDLVSSLSPSLESSSQVEP GTDRKSTGFETLVVTSEDGI TKIMFNRPKKKNAINTEMYH EIMRALKAASKDDSIITVLT GNGDYYSSGNDLTNFTDIPP GGVEEKAKNNAVLLREFVGC FIDFPKPLIAVVNGPAVGIS VTLLGLFDAVYASDRATFH
Tissue Specificity Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells.
Function Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
Protein Name Enoyl-Coa Delta Isomerase 2
Drs-1
Delta(3 -Delta(2-Enoyl-Coa Isomerase
D3 -D2-Enoyl-Coa Isomerase
Diazepam-Binding Inhibitor-Related Protein 1
Dbi-Related Protein 1
Dodecenoyl-Coa Isomerase
Hepatocellular Carcinoma-Associated Antigen 88
Peroxisomal 3 -2-Trans-Enoyl-Coa Isomerase
Peci
Renal Carcinoma Antigen Ny-Ren-1
Database Links Reactome: R-HSA-390247 O75521-2
Reactome: R-HSA-9033241 O75521-2
Cellular Localisation Isoform 1: Mitochondrion
Isoform 2: Peroxisome Matrix
Alternative Antibody Names Anti-Enoyl-Coa Delta Isomerase 2 antibody
Anti-Drs-1 antibody
Anti-Delta(3 -Delta(2-Enoyl-Coa Isomerase antibody
Anti-D3 -D2-Enoyl-Coa Isomerase antibody
Anti-Diazepam-Binding Inhibitor-Related Protein 1 antibody
Anti-Dbi-Related Protein 1 antibody
Anti-Dodecenoyl-Coa Isomerase antibody
Anti-Hepatocellular Carcinoma-Associated Antigen 88 antibody
Anti-Peroxisomal 3 -2-Trans-Enoyl-Coa Isomerase antibody
Anti-Peci antibody
Anti-Renal Carcinoma Antigen Ny-Ren-1 antibody
Anti-ECI2 antibody
Anti-DRS1 antibody
Anti-HCA88 antibody
Anti-PECI antibody

Information sourced from Uniprot.org

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