Anti-EBP antibody (50-150) (STJ117045)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EBP (50-150) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EBP |
| Gene ID | 10682 |
| Uniprot ID | EBP_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | SGRAAVVPLGTWRRLSLCWF AVCGFIHLVIEGWFVLYYED LLGDQAFLSQLWKEYAKGDS RYILGDNFTVCMETITACLW GPLSLWVVIAFLRQHPLRFI L |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human EBP (NP_006570.1). |
Additional Info
| Function | Isomerase that catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers a catalytic step in the postlanosterol biosynthesis of cholesterol. Component of the microsomal antiestrogen binding site (AEBS), a multiproteic complex at the ER membrane that consists of an association between EBP and 7-dehydrocholesterol reductase/DHCR7. This complex is responsible for cholesterol-5,6-epoxide hydrolase (ChEH) activity, which consists in the hydration of cholesterol-5,6-epoxides (5,6-EC) into cholestane-3beta,5alpha,6beta-triol (CT). The precise role of each component of this complex has not been described yet. |
| Protein Name | 3-Beta-Hydroxysteroid-Delta(8 -Delta(7-IsomeraseCholestenol Delta-IsomeraseCholesterol-5 -6-Epoxide Hydrolase Subunit EbpDelta(8-Delta(7 Sterol IsomeraseD8-D7 Sterol IsomeraseEmopamil-Binding Protein |
| Database Links | Reactome: R-HSA-6807047Reactome: R-HSA-6807062 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinNucleus EnvelopeCytoplasmic VesicleDuring InterphaseDetected On The Endoplasmic Reticulum And The Nuclear EnvelopeDuring MitosisDetected On Cytoplasmic Vesicles |
| Alternative Antibody Names | Anti-3-Beta-Hydroxysteroid-Delta(8 -Delta(7-Isomerase antibodyAnti-Cholestenol Delta-Isomerase antibodyAnti-Cholesterol-5 -6-Epoxide Hydrolase Subunit Ebp antibodyAnti-Delta(8-Delta(7 Sterol Isomerase antibodyAnti-D8-D7 Sterol Isomerase antibodyAnti-Emopamil-Binding Protein antibodyAnti-EBP antibody |
Information sourced from Uniprot.org