Anti-EBF1 antibody (500-591) (STJ114934)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EBF1 (500-591) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EBF1 |
| Gene ID | 1879 |
| Uniprot ID | COE1_HUMAN |
| Immunogen Region | 500-591 |
| Immunogen Sequence | GGSPTFLNGSAANSPYAIVP SSPTMASSTSLPSNCSSSSG IFSFSPANMVSAVKQKSAFA PVVRPQTSPPPTCTSTNGNS LQAISGMIVPPM |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 500-591 of human EBF1 (NP_076870.1). |
Additional Info
| Function | Key pioneer transcription factor of B-cell specification and commitment. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold. (Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells. In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency. |
| Protein Name | Transcription Factor Coe1O/E-1Oe-1Early B-Cell Factor |
| Database Links | Reactome: R-HSA-381340Reactome: R-HSA-9752946 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor Coe1 antibodyAnti-O/E-1 antibodyAnti-Oe-1 antibodyAnti-Early B-Cell Factor antibodyAnti-EBF1 antibodyAnti-COE1 antibodyAnti-EBF antibody |
Information sourced from Uniprot.org