Confocal imaging of C2C12 cells usingย EB3/MAPRE3 Rabbit monoclonal antibody (STJ11103122, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-EB3/MAPRE3 antibody (182-281) [S2MR] (STJ11103122)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103122
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General Information

Short DescriptionRabbit monoclonal EB3/MAPRE3 (182-281) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS2MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAPRE3
Gene ID22924
Uniprot IDMARE3_HUMAN
Immunogen Region182-281
Immunogen SequenceCILRKNPPSARNGGHETDAQ ILELNQQLVDLKLTVDGLEK ERDFYFSKLRDIELICQEHE SENSPVISGIIGILYATEEG FAPPEDDEIEEHQQEDQDEY
SpecificityA synthetic peptide corresponding to a sequence within amino acids 182-281 of human EB3/MAPRE3 (Q9UPY8).

Additional Info

Tissue Specificity Predominantly expressed in brain and muscle.
Function Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules.
Protein Name Microtubule-Associated Protein Rp/Eb Family Member 3
Eb1 Protein Family Member 3
Ebf3
End-Binding Protein 3
Eb3
Rp3
Database Links
Cellular Localisation Cytoplasm
Cytoskeleton
Associated With The Microtubule Network
Detected At The Plus End Of Microtubules
Alternative Antibody Names Anti-Microtubule-Associated Protein Rp/Eb Family Member 3 antibody
Anti-Eb1 Protein Family Member 3 antibody
Anti-Ebf3 antibody
Anti-End-Binding Protein 3 antibody
Anti-Eb3 antibody
Anti-Rp3 antibody
Anti-MAPRE3 antibody

Information sourced from Uniprot.org

Citations

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