Anti-EB1 antibody (C-Term) [KT51] (STJA0041194)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenClone KT51 was generated from a GST fusion protein containing amino acids from the C-terminal region of mouse EB1. This sequence is highly conserved in human and rat EB1.
General Information
| Short Description | Rat monoclonal anti-EB1 (C-Term) for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Rat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | KT51 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Purification | Protein G Purified |
| Dilution Range | WB 1:1000ICC 1:200 |
| Formulation | PBS + 0.05% NaN3 |
| Storage Instruction | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at-20°C C or-80°C C, while keeping a working aliquot stored at 4°C C for short term. Avoid freeze/t |
Target Information
| Gene Symbol | Mapre1 |
| Gene ID | 13589 |
| Uniprot ID | MARE1_MOUSE |
| Immunogen | Clone KT51 was generated from a GST fusion protein containing amino acids from the C-terminal region of mouse EB1. This sequence is highly conserved in human and rat EB1. |
| Immunogen Region | C-Term |
| Immunogen Sequence | Mouse |
| Specificity | The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells. |
Additional Info
| Tissue Specificity | Expressed within the midpiece of sperm tail (at protein level). |
| Post Translational Modifications | Acetylation at Lys-220 by KAT2B/PCAF promotes dynamic kinetochore-microtubule interactions in early mitosis. Crotonylated by KAT5 during mitosis, promoting astral microtubule plasticity and dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation, thereby ensuring accurate spindle positioning in mitosis. Decrotonylated by HDAC3. |
| Function | Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains. Promotes cytoplasmic microtubule nucleation and elongation. Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation. Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers). Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2. Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation. May play a role in cell migration. |
| Protein Name | Microtubule-Associated Protein Rp/Eb Family Member 1Apc-Binding Protein Eb1End-Binding Protein 1Eb1 |
| Database Links | Reactome: R-MMU-141444Reactome: -MMU-2467813Reactome: -MMU-2500257Reactome: -MMU-2565942Reactome: -MMU-380259Reactome: -MMU-380270Reactome: -MMU-380284Reactome: -MMU-380320Reactome: -MMU-5620912Reactome: -MMU-5663220Reactome: -MMU-68877Reactome: -MMU-8852276Reactome: -MMU-8854518Reactome: -MMU-9648025 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleSpindle PoleAssociated With The Microtubule Network At The Growing Distal Tip Of MicrotubulesIn Addition To Localizing To Microtubule Plus-EndsAlso Exhibits Some Localization Along The Length Of The MicrotubulesAlso Enriched At The Centrosome |
| Alternative Antibody Names | Anti-Microtubule-Associated Protein Rp/Eb Family Member 1 antibodyAnti-Apc-Binding Protein Eb1 antibodyAnti-End-Binding Protein 1 antibodyAnti-Eb1 antibodyAnti-Mapre1 antibody |
Information sourced from Uniprot.org