| Short Description : | Rabbit polyclonal EARS2 (42-220) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EARS2 |
| Gene ID: | 124454 |
| Uniprot ID: | SYEM_HUMAN |
| Immunogen Region: | 42-220 |
| Immunogen Sequence: | APSPTGFLHLGGLRTALYNY IFAKKYQGSFILRLEDTDQT RVVPGAAENIEDMLEWAGIP PDESPRRGGPAGPYQQSQRL ELYAQATEALLKTGAAYPCF CSPQRLELLKKEALRNHQTP RYDNRCRNMSQEQVAQKLAK DPKPAIRFRLEQVVPAFQDL VYGWNRHEVASVEGDPVIM |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-220 of human EARS2 (NP_001295140.1). |
| Function | Non-discriminating glutamyl-tRNA synthetase that catalyzes aminoacylation of both mitochondrial tRNA(Glu) and tRNA(Gln) and participates in RNA aminoacylation for mitochondrial protein translation. Attachs glutamate to tRNA(Glu) or tRNA(Gln) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) or tRNA(Gln). In vitro, cytoplasmic tRNA(Gln) is slightly glutamylated, but with low activity. |
| Protein Name | Nondiscriminating Glutamyl-Trna Synthetase Ears2 - MitochondrialGlutamate--Trna(Gln Ligase Ears2 - MitochondrialGlutamyl-Trna SynthetaseGlursMitochondrial Glutamyl-Trna SynthetaseMtglurs |
| Database Links | Reactome: R-HSA-379726 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Nondiscriminating Glutamyl-Trna Synthetase Ears2 - Mitochondrial antibodyAnti-Glutamate--Trna(Gln Ligase Ears2 - Mitochondrial antibodyAnti-Glutamyl-Trna Synthetase antibodyAnti-Glurs antibodyAnti-Mitochondrial Glutamyl-Trna Synthetase antibodyAnti-Mtglurs antibodyAnti-EARS2 antibodyAnti-KIAA1970 antibody |
Information sourced from Uniprot.org
