Anti-Early Endosome Antigen 1 antibody (N-Term) [M347] (STJA0041193)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenClone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1.
General Information
| Short Description | Mouse monoclonal anti-Early Endosome antigen 1 (N-Term) for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M347 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Protein A Purified |
| Dilution Range | WB 1:1000ICC 1:50 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | EEA1 |
| Gene ID | 8411 |
| Uniprot ID | EEA1_HUMAN |
| Immunogen | Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1. |
| Immunogen Region | N-Term |
| Immunogen Sequence | Human |
| Specificity | This antibody detects a 180 kDa* protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles. |
Additional Info
| Function | Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. |
| Protein Name | Early Endosome Antigen 1Endosome-Associated Protein P162Zinc Finger Fyve Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-168138 |
| Cellular Localisation | CytoplasmEarly Endosome MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Early Endosome Antigen 1 antibodyAnti-Endosome-Associated Protein P162 antibodyAnti-Zinc Finger Fyve Domain-Containing Protein 2 antibodyAnti-EEA1 antibodyAnti-ZFYVE2 antibody |
Information sourced from Uniprot.org