Anti-E4F1 antibody (STJ197544)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human E4F1
General Information
| Short Description | Rabbit polyclonal anti-Transcription factor E4F1 for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | E4F1 |
| Gene ID | 1877 |
| Uniprot ID | E4F1_HUMAN |
| Immunogen | Synthesized peptide derived from the human E4F1 |
| Specificity | This antibody detects endogenous levels of human E4F1 |
Additional Info
| Post Translational Modifications | Proteolytic cleavage produces a 50 kDa N-terminal peptide (p50E4F) which has a DNA-binding activity and activates transcription in presence of the adenoviral E1A protein. The major full-length protein (p120E4F) functions as a repressor of transcription. Phosphorylated.p120E4F and p50E4F are both phosphorylated. Phosphorylation is cell cycle-dependent and differentially regulates DNA-binding activity and function of both forms. May be sumoylated by UBE2I upon interaction with CDKN2A. |
| Function | May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription. Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes. |
| Protein Name | Transcription Factor E4f1E4f Transcription Factor 1Putative E3 Ubiquitin-Protein Ligase E4f1Ring-Type E3 Ubiquitin Transferase E4f1Transcription Factor E4fP120e4fP50e4f |
| Database Links | |
| Cellular Localisation | NucleusNucleoplasmCytoplasmA Small Fraction Is Detected In The CytoplasmExcluded From The Nucleolus Where It Is Targeted Upon Cdkn2a OverexpressionLocalizes To The Mitotic Spindle During Embryogenesis |
| Alternative Antibody Names | Anti-Transcription Factor E4f1 antibodyAnti-E4f Transcription Factor 1 antibodyAnti-Putative E3 Ubiquitin-Protein Ligase E4f1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase E4f1 antibodyAnti-Transcription Factor E4f antibodyAnti-P120e4f antibodyAnti-P50e4f antibodyAnti-E4F1 antibodyAnti-E4F antibody |
Information sourced from Uniprot.org