Anti-E2F4 antibody (Internal) (STJ72199)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-E2F4 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 48kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 44.0kDa according to NP_001941.2). Recommended concentration: 1-3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | E2F4 |
| Gene ID | 1874 |
| Uniprot ID | E2F4_HUMAN |
| Accession Number | NP_001941.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | DPTGVLELPKELSE |
Additional Info
| Post Translational Modifications | Differentially phosphorylated in vivo. |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis. |
| Protein Name | Transcription Factor E2f4E2f-4 |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-1538133Reactome: R-HSA-2173796Reactome: R-HSA-6804114Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69656 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2f4 antibodyAnti-E2f-4 antibodyAnti-E2F4 antibody |
Information sourced from Uniprot.org