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Anti-E2F1 antibody (100-170 aa) (STJ98642)

Western blot analysis ofmouse braincells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, E2F-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-heart tissue. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-heart tissue. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-E2F1 antibody (100-170 aa) (STJ98642)

SKU:

STJ98642

£46.00 - £226.00

Current Stock:

General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	IF/WB/IHC/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Transcription factor E2F1 (100-170 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IF 1:50-200 WB 1:500-2000 ELISA 1:10000-20000 IHC 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	E2F1
Gene ID:	1869
Uniprot ID:	E2F1_HUMAN
Immunogen Region:	100-170 aa
Specificity:	The antibody detects endogenous E2F-1 protein
Immunogen:	Synthetic peptide from the human protein at the amino acid range 100-170

Function	Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Directly activates transcription of PEG10. Positively regulates transcription of RRP1B.
Protein Name	Transcription Factor E2f1 E2f-1 Pbr3 Retinoblastoma-Associated Protein 1 Rbap-1 Retinoblastoma-Binding Protein 3 Rbbp-3 Prb-Binding Protein E2f-1
Database Links	Reactome: R-HSA-111448 Reactome: R-HSA-113501 Reactome: R-HSA-1362277 Reactome: R-HSA-1362300 Reactome: R-HSA-139915 Reactome: R-HSA-1912408 Reactome: R-HSA-2559580 Reactome: R-HSA-2559585 Reactome: R-HSA-6804116 Reactome: R-HSA-68911 Reactome: R-HSA-69202 Reactome: R-HSA-69205 Reactome: R-HSA-69231 Reactome: R-HSA-69656 Reactome: R-HSA-8953750 Reactome: R-HSA-9616222 Reactome: R-HSA-9661069
Cellular Localisation	Nucleus
Alternative Antibody Names	Anti-Transcription Factor E2f1 antibody Anti-E2f-1 antibody Anti-Pbr3 antibody Anti-Retinoblastoma-Associated Protein 1 antibody Anti-Rbap-1 antibody Anti-Retinoblastoma-Binding Protein 3 antibody Anti-Rbbp-3 antibody Anti-Prb-Binding Protein E2f-1 antibody Anti-E2F1 antibody Anti-RBBP3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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