Anti-DZIP1 antibody (73-123 aa) (STJ194668)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human DZIP1 at the amino acid range 73-123
General Information
| Short Description | Rabbit polyclonal anti-Cilium assembly protein DZIP1 (73-123 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DZIP1 |
| Gene ID | 22873 |
| Uniprot ID | DZIP1_HUMAN |
| Immunogen | Synthesized peptide derived from the human DZIP1 at the amino acid range 73-123 |
| Immunogen Region | 73-123 aa |
| Specificity | This antibody detects endogenous levels of DZIP1 at Human/Mouse |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-226 by PLK1 before mitosis prevents interaction with PCM1 and localization to centriolar satellites. Thereby, it negatively regulates the localization of the BBSome to centriolar satellites. |
| Function | Molecular adapter that recruits protein complexes required for cilium assembly and function to the cilium basal body. At the exit of mitosis, localizes to the basal body and ciliary base of the forming primary cilium where it recruits and activates RAB8A to direct vesicle-mediated transport of proteins to the cilium. Also recruits the BBSome, a complex involved in cilium biogenesis, by bridging it to PCM1 at the centriolar satellites of the cilium. It is also required for the recruitment to the cilium basal body of the intraflagellar transport (IFT) machinery as well as the ciliary appendage proteins CEP164 and NINEIN. Functions as a regulator of Hedgehog signaling both through its role in cilium assembly but also probably through its ability to retain GLI3 within the cytoplasm. It is involved in spermatogenesis through its role in organization of the basal body and assembly of the sperm flagellum. Also indirectly involved in heart development through its function in ciliogenesis. |
| Protein Name | Cilium Assembly Protein Dzip1Daz-Interacting Protein 1/2Daz-Interacting Zinc Finger Protein 1 |
| Database Links | Reactome: R-HSA-5632684 |
| Cellular Localisation | CytoplasmCytoskeletonCilium Basal BodyMicrotubule Organizing CenterCentrosomeCentriolar SatelliteCentrioleNucleusNucleus SpeckleLocalizes To The Centriole In Cells Lacking Cilia And To The Cilium Basal Body In Ciliated CellsAt The Exit Of MitosisWhen The Primary Cilium Is Reassembled In Daughter CellsLocalizes At The Mother Centriole That Acts As The Basal Body Of The Assembling Primary Cilium And Also Accumulates At The Ciliary Base That Constitutes A Diffusion Barrier For Ciliary Proteins |
| Alternative Antibody Names | Anti-Cilium Assembly Protein Dzip1 antibodyAnti-Daz-Interacting Protein 1/2 antibodyAnti-Daz-Interacting Zinc Finger Protein 1 antibodyAnti-DZIP1 antibodyAnti-DZIP antibodyAnti-DZIP2 antibodyAnti-KIAA0996 antibody |
Information sourced from Uniprot.org