Anti-Dysferlin antibody [R02-4B1] (STJA0035985)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Dysferlin
General Information
| Short Description | Rabbit monoclonal anti-Dysferlin for use in WB, IHC-P, ICC and IF in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-4B1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DYSF |
| Gene ID | 8291 |
| Uniprot ID | DYSF_HUMAN |
| Immunogen | A synthesized peptide derived from human Dysferlin |
Additional Info
| Tissue Specificity | Expressed in skeletal muscle, myoblast, myotube and in the syncytiotrophoblast (STB) of the placenta (at protein level). Ubiquitous. Highly expressed in skeletal muscle. Also found in heart, brain, spleen, intestine, placenta and at lower levels in liver, lung, kidney and pancreas. |
| Function | Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. |
| Protein Name | DysferlinDystrophy-Associated Fer-1-Like ProteinFer-1-Like Protein 1 |
| Database Links | Reactome: R-HSA-445355 |
| Cellular Localisation | Cell MembraneSarcolemmaSingle-Pass Type Ii Membrane ProteinCytoplasmic Vesicle MembraneColocalizesDuring Muscle DifferentiationWith Bin1 In The T-Tubule System Of Myotubules And At The Site Of Contact Between Two Myotubes Or A Myoblast And A MyotubeWounding Of Myotubes Led To Its Focal Enrichment To The Site Of Injury And To Its Relocalization In A Ca(2+)-Dependent Manner Toward The Plasma MembraneColocalizes With AhnakAhnak2 And Parvb At The Sarcolemma Of Skeletal MuscleDetected On The Apical Plasma Membrane Of The SyncytiotrophoblastReaches The Plasmma Membrane Through A Caveolin-Independent MechanismRetained By Caveolin At The Plasmma MembraneWith Cacna1s In The T-Tubule System Of MyotubulesAccumulates And Colocalizes With Fusion Vesicles At The Sarcolemma Disruption Sites |
| Alternative Antibody Names | Anti-Dysferlin antibodyAnti-Dystrophy-Associated Fer-1-Like Protein antibodyAnti-Fer-1-Like Protein 1 antibodyAnti-DYSF antibodyAnti-FER1L1 antibody |
Information sourced from Uniprot.org