• Western blot analysis of extracts of various cell lines, using Dynein intermediate chain 1 antibody (STJ11103528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Dynein intermediate chain 1 antibody (1-200) [S8MR] (STJ11103528)

SKU:
STJ11103528

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Dynein intermediate chain 1 (1-200) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNAI1
Gene ID: 27019
Uniprot ID: DNAI1_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Dynein intermediate chain 1 (Q9UI46).
Immunogen Sequence: MIPASAKAPHKQPHKQSISI GRGTRKRDEDSGTEVGEGTD EWAQSKATVRPPDQLELTDA ELKEEFTRILTANNPHAPQN IVRYSFKEGTYKPIGFVNQL AVHYTQVGNLIPKDSDEGRR QHYRDELVAGSQESVKVISE TGNLEEDEEPKELETEPGSQ TDVPAAGAAEKVTEEELMTP KQPKERKLTNQFNFSERASQ
Tissue Specificity Expressed in respiratory ciliated cells (at protein level).
Function Part of the dynein complex of respiratory cilia.
Protein Name Dynein Axonemal Intermediate Chain 1
Axonemal Dynein Intermediate Chain 1
Cellular Localisation Dynein Axonemal Particle
Cytoplasm
Cytoskeleton
Cilium Axoneme
Alternative Antibody Names Anti-Dynein Axonemal Intermediate Chain 1 antibody
Anti-Axonemal Dynein Intermediate Chain 1 antibody
Anti-DNAI1 antibody

Information sourced from Uniprot.org

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