Anti-WAS antibody (1-100) [S8MR] (STJ11102268)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal WASP (1-100) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S8MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WAS |
| Gene ID | 7454 |
| Uniprot ID | WASP_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MSGGPMGGRPGGRGAPAVQQ NIPSTLLQDHENQRLFEMLG RKCLTLATAVVQLYLALPPG AEHWTKEHCGAVCFVKDNPQ KSYFIRLYGLQAGRLLWEQE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WASP (P42768). |
Additional Info
| Tissue Specificity | Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen. |
| Post Translational Modifications | Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation. |
| Function | Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). |
| Protein Name | Actin Nucleation-Promoting Factor WasWiskott-Aldrich Syndrome ProteinWasp |
| Database Links | Reactome: R-HSA-202433Reactome: R-HSA-2029482Reactome: R-HSA-5663213Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013409Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonNucleus |
| Alternative Antibody Names | Anti-Actin Nucleation-Promoting Factor Was antibodyAnti-Wiskott-Aldrich Syndrome Protein antibodyAnti-Wasp antibodyAnti-WAS antibodyAnti-IMD2 antibody |
Information sourced from Uniprot.org