Anti-DYKDDDDK Epitope Tag antibody (DYKDDDDKC) [AT3G6] (STJA0041877)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenSynthetic peptide (DYKDDDDKC)-KLH
General Information
| Short Description | Mouse monoclonal anti-DYKDDDDK Epitope Tag (DYKDDDDKC) for use in ELISA and WB in DDDDK Tag samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB |
| Host | Mouse |
| Reactivity | DDDDK Tag |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | AT3G6 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | By protein-A affinity chromatography |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen | Synthetic peptide (DYKDDDDKC)-KLH |
| Immunogen Region | DYKDDDDKC |
Additional Info
| Background | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins with in living cells. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |
Information sourced from Uniprot.org