Anti-DXO antibody (STJA0007157)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human DOM3Z
General Information
| Short Description | Rabbit polyclonal anti-Decapping and exoribonuclease protein for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DXO |
| Gene ID | 1797 |
| Uniprot ID | DXO_HUMAN |
| Immunogen | Synthesized peptide derived from human DOM3Z |
| Specificity | This antibody detects endogenous levels of DOM3Z at Human, Mouse, Rat |
Additional Info
| Function | Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to environmental stress. Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation. Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control. Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1). In contrast to canonical decapping enzymes DCP2 and NUDT16, which cleave the cap within the triphosphate linkage, the decapping activity releases the entire cap structure GpppN and a 5'-end monophosphate RNA. Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates. Exhibits decapping activity towards FAD-capped RNAs. Exhibits decapping activity towards dpCoA-capped RNAs in vitro. |
| Protein Name | Decapping And Exoribonuclease ProteinDxo5'-3' Exoribonuclease DxoDom-3 Homolog ZNad-Capped Rna Hydrolase DxoDenadding Enzyme Dxo |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Decapping And Exoribonuclease Protein antibodyAnti-Dxo antibodyAnti-5'-3' Exoribonuclease Dxo antibodyAnti-Dom-3 Homolog Z antibodyAnti-Nad-Capped Rna Hydrolase Dxo antibodyAnti-Denadding Enzyme Dxo antibodyAnti-DXO antibodyAnti-DOM3L antibodyAnti-DOM3Z antibodyAnti-NG6 antibody |
Information sourced from Uniprot.org