• Immunohistochemistry analysis of paraffin-embedded rat ovary using DVL3 Rabbit polyclonal antibody (STJ11103537) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using DVL3 Rabbit polyclonal antibody (STJ11103537) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using DVL3 Rabbit polyclonal antibody (STJ11103537) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using DVL3 Rabbit polyclonal antibody (STJ11103537) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DVL3 antibody (STJ11103537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DVL3 antibody (580-650) (STJ11103537)

SKU:
STJ11103537

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DVL3 (580-650) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DVL3
Gene ID: 1857
Uniprot ID: DVL3_HUMAN
Immunogen Region: 580-650
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 580-650 of human DVL3 (NP_004414.3).
Immunogen Sequence: RSGSDRRKEKDPKAGDSKSG GSGSESDHTTRSSLRGPRER APSERSGPAASEHSHRSHHS LASSLRSHHTH
Post Translational Modifications Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Phosphorylated by CSNK1D. Arginine methylation may function as a switch in regulation of function in Wnt signaling.
Function Involved in the signal transduction pathway mediated by multiple Wnt genes.
Protein Name Segment Polarity Protein Dishevelled Homolog Dvl-3
Dishevelled-3
Dsh Homolog 3
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-201688
Reactome: R-HSA-4086400
Reactome: R-HSA-4641258
Reactome: R-HSA-4641262
Reactome: R-HSA-5368598
Reactome: R-HSA-5663220
Reactome: R-HSA-9673324
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Segment Polarity Protein Dishevelled Homolog Dvl-3 antibody
Anti-Dishevelled-3 antibody
Anti-Dsh Homolog 3 antibody
Anti-DVL3 antibody
Anti-KIAA0208 antibody

Information sourced from Uniprot.org

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