| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Pig/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-dUTPase/DUT (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-0.1-0.3µg/mlIHC-5µg/mlELISA-antibody detection limit dilution 1:16000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
DUT |
| Gene ID: |
1854 |
| Uniprot ID: |
DUT_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_001020419.1; NP_001939.1; NP_001020420.1 |
| Specificity: |
This antibody is expected to recognize all reported isoforms (NP_001020419.1; NP_001939.1; NP_001020420.1). |
| Immunogen Sequence: |
QLRFARLSEHATAPT |
| Post Translational Modifications | Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated. |
| Function | Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development. |
| Protein Name | Deoxyuridine 5'-Triphosphate Nucleotidohydrolase - MitochondrialDutpaseDutp Pyrophosphatase |
| Database Links | Reactome: R-HSA-499943 P33316-2 |
| Cellular Localisation | Isoform 2: NucleusIsoform 3: Mitochondrion |
| Alternative Antibody Names | Anti-Deoxyuridine 5'-Triphosphate Nucleotidohydrolase - Mitochondrial antibodyAnti-Dutpase antibodyAnti-Dutp Pyrophosphatase antibodyAnti-DUT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
