Anti-DUTPase antibody [R89-6Y-1] (STJA0035635)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human DUT
General Information
| Short Description | Rabbit monoclonal anti-DUTPase for use in WB, ICC, IF and FC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/FC |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R89-6Y-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200FC 1:50 |
| Formulation | Liquid in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DUT |
| Gene ID | 1854 |
| Uniprot ID | DUT_HUMAN |
| Immunogen | A synthesized peptide derived from human DUT |
Additional Info
| Tissue Specificity | Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level). |
| Post Translational Modifications | Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated. |
| Function | Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development. |
| Protein Name | Deoxyuridine 5'-Triphosphate Nucleotidohydrolase - MitochondrialDutpaseDutp Pyrophosphatase |
| Database Links | Reactome: R-HSA-499943 P33316-2 |
| Cellular Localisation | Isoform 2: NucleusIsoform 3: Mitochondrion |
| Alternative Antibody Names | Anti-Deoxyuridine 5'-Triphosphate Nucleotidohydrolase - Mitochondrial antibodyAnti-Dutpase antibodyAnti-Dutp Pyrophosphatase antibodyAnti-DUT antibody |
Information sourced from Uniprot.org