• Immunofluorescence analysis of C6 cells using DUSP6 Rabbit polyclonal antibody (STJ23447) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using DUSP6 Rabbit polyclonal antibody (STJ23447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DUSP6 antibody (STJ23447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DUSP6 antibody (302-381) (STJ23447)

SKU:
STJ23447

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DUSP6 (302-381) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DUSP6
Gene ID: 1848
Uniprot ID: DUS6_HUMAN
Immunogen Region: 302-381
Immunogen: Recombinant fusion protein containing a sequence within amino acids 302-381 of human DUSP6 (NP_001937.2).
Immunogen Sequence: TVTVAYLMQKLNLSMNDAYD IVKMKKSNISPNFNFMGQLL DFERTLGLSSPCDNRVPAQQ LYFTTPSNQNVYQVDSLQST
Tissue Specificity Expressed in keratinocytes (at protein level).
Function Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain. Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors.
Protein Name Dual Specificity Protein Phosphatase 6
Dual Specificity Protein Phosphatase Pyst1
Mitogen-Activated Protein Kinase Phosphatase 3
Map Kinase Phosphatase 3
Mkp-3
Database Links Reactome: R-HSA-112409
Reactome: R-HSA-202670
Reactome: R-HSA-5675221
Reactome: R-HSA-9652817
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Dual Specificity Protein Phosphatase 6 antibody
Anti-Dual Specificity Protein Phosphatase Pyst1 antibody
Anti-Mitogen-Activated Protein Kinase Phosphatase 3 antibody
Anti-Map Kinase Phosphatase 3 antibody
Anti-Mkp-3 antibody
Anti-DUSP6 antibody
Anti-MKP3 antibody
Anti-PYST1 antibody

Information sourced from Uniprot.org

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