• Western blot analysis of various lysates using DUSP12 Rabbit polyclonal antibody (STJ115212) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DUSP12 antibody (130-270) (STJ115212)

SKU:
STJ115212

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DUSP12 (130-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DUSP12
Gene ID: 11266
Uniprot ID: DUS12_HUMAN
Immunogen Region: 130-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-270 of human DUSP12 (NP_009171.1).
Immunogen Sequence: LMKTDQLPFEKAYEKLQILK PEAKMNEGFEWQLKLYQAMG YEVDTSSAIYKQYRLQKVTE KYPELQNLPQELFAVDPTTV SQGLKDEVLYKCRKCRRSLF RSSSILDHREGSGPIAFAHK RMTPSSMLTTGRQAQCTSYF I
Tissue Specificity Ubiquitous, highest expression in spleen, testis, ovary, and peripheral blood leukocytes and lower expression in liver and lung.
Function Dual specificity phosphatase.can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro). Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates.
Protein Name Dual Specificity Protein Phosphatase 12
Dual Specificity Tyrosine Phosphatase Yvh1
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Primarily Nuclear
Detected In A Mesh-Like Pattern In The Cytosol
Alternative Antibody Names Anti-Dual Specificity Protein Phosphatase 12 antibody
Anti-Dual Specificity Tyrosine Phosphatase Yvh1 antibody
Anti-DUSP12 antibody

Information sourced from Uniprot.org

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