Anti-Drosha antibody [R01-9I2] (STJA0036421)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Drosha
General Information
| Short Description | Rabbit monoclonal anti-Drosha for use in WB, IHC-P, ICC, IF and FC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/FC |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-9I2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DROSHA |
| Gene ID | 29102 |
| Uniprot ID | RNC_HUMAN |
| Immunogen | A synthesized peptide derived from human Drosha |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Degraded by autophagy in response to neuronal activity in motor neurons. |
| Function | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons. |
| Protein Name | Ribonuclease 3Protein DroshaRibonuclease IiiRnase IiiP241 |
| Database Links | Reactome: R-HSA-203927 |
| Cellular Localisation | NucleusNucleolusCytoplasmA Fraction Is Translocated To The Nucleolus During The S Phase Of The Cell CycleLocalized In Gw Bodies (Gwbs)Also Known As P-Bodies |
| Alternative Antibody Names | Anti-Ribonuclease 3 antibodyAnti-Protein Drosha antibodyAnti-Ribonuclease Iii antibodyAnti-Rnase Iii antibodyAnti-P241 antibodyAnti-DROSHA antibodyAnti-RN3 antibodyAnti-RNASE3L antibodyAnti-RNASEN antibody |
Information sourced from Uniprot.org